Table 3. Relative binding affinity of cellular mRNAs with TS protein.

RNA	IC50 (nM)
TS:N25	0.08
eIF2B	0.10
ATK	0.12
TS:N25AS	>10

The binding affinity of ATK and eIF2B RNA for human recombinant TS was determined by the RNA gel shift assay as described in Materials and Methods. Each value represents the mean of between three and five experiments and the SE for each experiment was <10%.