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. 2000 Nov 1;28(21):4299–4305. doi: 10.1093/nar/28.21.4299

Figure 5.

Figure 5

Assembly of an archaeal–human hybrid complex. (A) Schematic representation of the expression constructs used. (B) Analysis of the protein expression profiles of bacterial cells harboring the constructs illustrated above. The insoluble and soluble fractions, together with GCE, are shown for the GST–mjF/mjE (lanes 1, 3 and 5) and GST–mjF/hsRPB7 (lanes 2, 4 and 6) expression strains. The cross-species bicistronic construct expressing GSTmjF and hsRPB7 expresses an archaeal–human hybrid complex (lane 6). Partial GST–mjF products present in this lane are indicated by an asterisk.