Figure 3. Deletion of endothelial Elavl1 enhances a subset of the endothelial translatome involved in immune regulation.

(A, inset) Differential gene expression (DESeq2, Log2 Fold Change) in response to low flow, comparing Elavl ECKO to Elavl WT. Red represents transcripts enriched >0.5 Log2 Fold Change in ECKO TRAP mRNA vs. WT TRAP mRNA. Blue represents transcripts depleted <0.5 Log2 Fold Change in the same comparison. (A) Genes from (red and blue from A, inset) were plotted on a volcano plot showing differential TRAP mRNA expression the low flow artery and the contralateral artery in wild-type mice. Pie charts show the proportion of transcripts down (left) or up (right) in TRAP mRNA in low flow response that are down (blue) or up (red) in ECKO response. (B) Enrichr analysis of top gene categories where the natural low flow response is enhanced by the deletion of Elavl1 (216 genes decreased in the low flow TRAP mRNA and further decreased by the loss of Elavl1, or 213 genes increased in low from TRAP mRNA and further increased by loss of Elavl1). Pink = Reactome, Blue = msigDB Hallmark, Crimson = GO Terms.