Figure 4. Characterization in vitro of 2024 Oropouche virus reassortment strain.
(A) Viral replication properties of OROV strain AM0088 and OROV strain BeAn 19991 in Vero CCL81 (left), Huh7 (center), and U-251 (right) cell lines. Cells were infected at a multiplicity of infection of 0.1. At indicated time points, samples were harvested, and titers were determined by focus-forming assay on Vero CCL81 cells. Statistical analyses were performed using the paired t-test. The median is presented by the middle line, and the upper and lower limits represent the 75th and 25th percentiles (whiskers). Statistical significance is ***p < 0.001, **p < 0.01, and *p < 0.05; ns, not significant. (B) Number of plaques formation by OROV strains AM0088 and BeAn 19991 at 36-, 48-, and 72- hpi in Vero CCL8 cells (n=12 wells). (C) Size of plaques by OROV strains AM0088 and BeAn 19991 at 36-, 48-, and 72- hpi in Vero CCL81 cells (n≥ 118 plaques).