Figure 5.

Kis regulates APPL localization and may act in a transcriptional complex with AICD and Tip60. (A) High-resolution confocal micrographs of terminal 6/7 boutons immunolabeled for APPL (green). HRP (magenta) was used to delineate presynaptic motor neurons. Scale bar = 5 µm. Right histogram shows the quantification of relative APPL fluorescence intensity. *** = p < 0.001 (B) Histogram showing appl transcript levels in kis mutants relative to w¹¹¹⁸ controls. (C) Representative eEJC traces recorded from muscle 6 of third instar larvae in response to administration of a suprathreshold stimulus to the presynaptic motor neuron. (D) Representative mEJC traces from larval body wall muscle 6. (E) Quantification of eEJC amplitudes (left), mEJC amplitudes (middle), and quantal content (right). ** = p < 0.01, *** = p < 0.001.