Figure 6.

Pharmacological inhibition of γ-secretase activity with L-685,458 impairs evoked neurotransmission and locomotion in controls but not in kis^LM27/kis^k13416 mutants. (A) Representative eEJC traces recorded from muscle 6 of third instar larvae in response to administration of a suprathreshold stimulus to the presynaptic motor neuron. C represents animals exposed to the vehicle, DMSO, while L represents animals exposed to 100 nM L-685,458 throughout development. (B) Quantification of eEJC amplitudes, mEJC amplitudes and frequency, and quantal content. * = p < 0.05 (C) wrMTrck quantification of larval crawling behavior on an agar arena for 30 s. C represents animals exposed to the vehicle, DMSO, while L represents animals exposed to 100 nM L-685,458 for 24 h. Histograms show average path length, average and maximum velocities, and body lengths per second. * = p < 0.05, ** = p < 0.01, *** = p < 0.001.