Fig. 5.

Gene editing to evaluate the importance of novel genes in pre-implantation development. A Transcript abundance of the novel gene ENSBTAG00000068261 in individual oocytes, eight-cell embryos and blastocysts. B Examples of sequences containing deletions of exon 2 of the ENSBTAG00000068261 novel gene. C Representative images of the embryos that arrested development at the morula stage (red asterisk) compared to control blastocysts (blue asterisk, all collected and imaged ~ 190hpf). Scale bar represents 100 µm