Figure 3.

OGE suppresses BRCA1 expression in HCC cells. (A,B) The three-dimensional scatterplot shows the relationship between abundance ratio weights and abundance ratios for individual identified proteins in the proteomic analysis of OGE (100 μg/mL) vs. untreated control (A) and OGE (200 μg/mL) vs. untreated control (B). The color represents the p-value of an individual protein. (C) GO biological process annotation of differentially down-regulatory proteins. The circle size indicates the number of mapping proteins in each process, and the color indicates the p-value of each process. (D) The expression of BRCA1 in both HepG2 and Mahlavu cells treated with the indicated doses of OGE was determined via Western blotting. Ratio, the relative protein level. β-actin, loading control. (E,F) The correlation between the expression of BRCA1 and OS (E) or PFS (F) of patients with HCC was analyzed using the Kaplan-Meier-plotter cancer database. The high versus low expression levels of BRCA1 mRNA were split by the median value. The follow-up threshold for patients was set as 60 months. Statistical analysis, log-rank test (E,F).