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. 2024 Aug 1;25(15):8424. doi: 10.3390/ijms25158424

Figure 4.

Figure 4

OGE activates the proteasomal degradation of BRCA1. (A) BRCA1 mRNA levels in HepG2 cells treated or untreated with OGE (200 μg/mL) for 24 h were determined using qPCR. GAPDH, 18S rRNA, and β-actin served as internal controls for gene expression. (B) After cycloheximide (CHX, 10 g/L) reaction for 1 h, HepG2 cells were treated or untreated with OGE (200 μg/mL) for the indicated times. BRCA1 expression in each experimental group was determined via Western blotting. (C) After reaction with MG132 (1 nM) or CQ (10 μM) for 30 min, HepG2 and Mahlavu cells were treated or untreated with OGE (200 μg/mL) for the indicated times. BRCA1 expression in each experimental group was determined via Western blotting. Ratio, the relative protein level. β-actin, loading control. Data are shown as the means ± SD. Statistical analysis, two-tailed unpaired Student’s t-test (A).