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. 2024 Aug 1;25(15):8424. doi: 10.3390/ijms25158424

Figure 6.

Figure 6

OGE attenuates the EMT and stemness features of HCC cells. (A,B) Transwell migration (A) and invasion assays (B) were performed using HepG2 cells treated with the indicated doses of OGE. Signal quantification of crystal violet extracts was measured via colorimetric analysis at 570 nm (n = 4). (C) The levels of EMT-related proteins in HepG2 and Mahlavu cells were determined via Western blotting. (D) A clonogenic assay using HepG2 cells treated with the indicated doses of OGE. Signal quantification was measured by colorimetric analysis at 570 nm (n = 3). (E) Spheroid formation assay of HepG2 cells treated with the indicated doses of OGE. Scale bar, 50 μm. (F) The levels of CSC-related proteins in HepG2 cells. Ratio, the relative protein level. β-actin, loading control. Data are shown as the means ± SD. One-way ANOVA with Tukey’s post hoc test (A,B,D,E). ★★, p < 0.01.