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. 2024 Aug 1;25(15):8409. doi: 10.3390/ijms25158409

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© 2024 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Cytotoxicity of the novel elastomeric ligatures. (A–C) Cell proliferation activity of L929 fibroblasts at 24, 48, and 72 h. (D) Fluorescence staining images of L929 viable cells cultured in the extracts from each group for 48 h (Scale bar = 100 μm). (E) Cell migration of L929 fibroblasts in each group. (F) Cell migration rate of L929 fibroblasts in each group. The elastomeric ligatures with 5% DMAHDM exhibited significant cytotoxicity compared to the other groups (p < 0.05), whereas those with 2% DMAHDM showed no cytotoxicity. The data were represented by Mean ± SD (n ≥ 3); different letters indicate significant differences (p < 0.05).