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. 2024 Jul 28;29(15):3556. doi: 10.3390/molecules29153556

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© 2024 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Expression of GLUC and CHIT class I and IV of M. indica induced by C. asianum and C. siamense. Uninoculated mango fruits were used as internal control. The days of analysis were 0, 1, 2, and 4 days post-inoculation (dpi) for RNA extraction and the assay by real-time quantitative reverse transcription polymerase chain reaction. Gene expression was normalized to the glyceraldehyde-3-phosphate dehydrogenase (GAPDH) gene [57] using the 2^−∆∆Ct method [58]. Data are shown as mean ± SD, analyzed by one-way ANOVA/Tukey test.

Expression of GLUC and CHIT class I and IV of M. indica induced by C. asianum and C. siamense. Uninoculated mango fruits were used as internal control. The days of analysis were 0, 1, 2, and 4 days post-inoculation (dpi) for RNA extraction and the assay by real-time quantitative reverse transcription polymerase chain reaction. Gene expression was normalized to the glyceraldehyde-3-phosphate dehydrogenase (GAPDH) gene [57] using the 2^−∆∆Ct method [58]. Data are shown as mean ± SD, analyzed by one-way ANOVA/Tukey test.