Table 3. Frameshift mutation repair is directed by single-stranded oligonucleotides.

Each reaction mixture contained the indicated amounts of plasmid and oligonucleotide. The extract used for these experiments came from HUH7 cells. The data represent the number of tetracycline resistant colonies per 10⁶ ampicillin resistant colonies generated from the same reaction and is the average of three independent experiments. Tet I is a chimeric oligonucleotide and Tet IX is a modified single-stranded oligonucleotide that are designed to insert a T residue at position 208 of pT^sΔ208. These oligonucleotides are equivalent to structures I and IX in Figure 2.