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. 2024 Jul 26;15:1430445. doi: 10.3389/fmicb.2024.1430445

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Copyright © 2024 Erickson, Ter Horst, Carlson, Falk and Kuo.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Alignments of the putative recombinant polerovirus sequences obtained in this study by RNAseq and confirmed by Sanger sequencing (colored red) with the potential parental CaPV1, TaPV, and TorCLV reference sequences (colored blue). The base reference sequence used was that of PaPV isolate 2. The double black line is a size marker for the aligned sequences, and the arrows beneath the line indicate the predicted open reading frame (ORF) translations. CaPV1, carrot polerovirus 1; TaPV, trachyspermum ammi polerovirus; TorCLV, Torilis crimson leaf virus; RSS, RNA interference silencing suppressor; Rap, replication associated protein; -1fs, -1 frameshifting site that enables P1 and P2 to be translated as a fusion protein; RdRp, RNA dependent RNA polymerase; CP, capsid protein; MP, movement protein; RTP, readthrough protein; r-t, ribosomal readthrough site that allows P3 and P5 to be translated as a fusion protein.