Skip to main content
. 2000 Nov 1;28(21):4350–4355. doi: 10.1093/nar/28.21.4350

Figure 4.

Figure 4

PNA-clamped PCR allows detection of a point mutation at a ratio of 1:100. Amplification in the presence (+ lanes) or absence (– lanes) of PNA was performed from mixed ratios of wild-type and mutant 3243 plasmid DNA. Lanes labeled W or M contain products amplified from wild-type or mutant plasmid, respectively. Lanes labeled H contain products amplified from heteroplasmic MELAS patient DNA. The negative image of the ethidium bromide stained gel is shown. DNA size standard (std) on the right is pBR322 digested with MspI.