Figure 6.

PNA-clamped PCR detects the T414G mutation in muscle DNA from older individuals. PCR product from the np 414 region of mtDNA was amplified in the absence of PNA from plasmid DNA (lanes M and W) or in the presence of PNA from DNA prepared from normal muscle biopsy (lanes labeled with age of individual). All products were digested with FokI, which cuts wild-type product to yield band labeled a (lane W) and mutant product to yield band labeled b (lane M). DNA size standard (std) on right is pBR322 digested with MspI.