Figure 1.

Analyses of purified human Polκ. (A) Purified human Polκ (200 ng) was analyzed by electrophoresis on a 10% SDS–polyacrylamide gel and visualized by silver staining. Protein size markers (lane M) are indicated on the left. (B) Purified human Polκ (40 ng) was analyzed by a western blot using a mouse monoclonal antibody against the His₆ tag. Protein size markers (lane M) are indicated on the right. (C) DNA polymerase assays were performed without (lane 1) or with (lane 2) purified human Polκ (0.2 ng, 2 fmol), using the 30mer DNA template, 5′-CCTTCTTCATTGGAACATACTTCTTCTTCC-3′, annealed with the 5′-³²P-labeled primer, 5′-GGAAGAAGAAGTATGTT-3′. DNA size markers in nucleotides are indicated on the right.