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Bypass of a template 8-oxoguanine (8-oxoG) by human Polκ. Standard DNA polymerase assays were performed with purified human Polκ (0.4 ng, 4 fmol) using the indicated DNA substrate containing a template 8-oxoG and a 32P-labeled 17mer primer. Polymerase reactions were carried out in the presence of a single deoxyribonucleoside triphosphate dATP (lane 2), dCTP (lane 3), dTTP (lane 4) or dGTP (lane 5), or all four dNTPs (lane 1). DNA size markers in nucleotides are indicated on the left.
