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Bypass of a template (–)-trans-anti-BPDE-N2-dG lesion by human Polκ. A 19mer primer was labeled with 32P at its 5′-end and annealed right before a template (–)-trans-anti-BPDE-N2-dG as shown on the right. Polymerase reactions were performed with 2 ng (20 fmol) of human Polκ in the presence of a single deoxyribonucleoside triphosphate dATP (lane 2), dCTP (lane 3), dTTP (lane 4) or dGTP (lane 5) or all four dNTPs (lane 1). DNA size markers in nucleotides are indicated on the left.
