Figure 7.

Comparative RT–PCR of AtRecQl1 to 4B and AtWRNexo using mRNA from different tissues (A) and a quantification of expression in flowers (B). (A) Each cDNA was amplified with 38 PCR cycles starting with reverse transcription of 10 ng mRNA from Arabidopsis rosette leaves, shoots, flowers or seedlings. (B) Each cDNA was amplified with 35 PCR cycles starting with reverse transcription of 10 ng mRNA from Arabidopsis flowers or a 1:10 or 1:100 dilution of this RT, respectively. (A and B) One-fifth of the PCR was subjected to gel electrophoresis and EtBr staining.