Fig. 5.
BSA-SeNPs inhibited hemin/erastin-induced ferroptotic damages in vitro. Hippocampal neuronal cells were co-treated with hemin or erastin and BSA-SeNPs for 6 h. (A–B) The relative living cell viability was imaged and detected by CCK-8 and after co-treatment with hemin and BSA-SeNPs (0.5 μM) or Na2SeO3 (0.5 μM) for 6 h. (C–D) The content of lipid ros was examined by flow cytometry. (E–F) The content of 4-HNE, MDA were respectively detected by cellular mouse 4-HNE (4-Hydroxynonenal) elisa kit and MDA elisa kit. ****P < 0.0001, **P < 0.01, *P < 0.05.