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. 1990 Jun 15;268(3):791–793. doi: 10.1042/bj2680791

Variation in intracellular P1P4-bis(5'-adenosyl) tetraphosphate (Ap4A) in virus-infected cells.

D J Johnston 1, C A Hart 1, A G McLennan 1
PMCID: PMC1131510  PMID: 2163623

Abstract

The effect of virus infection on the intracellular concentration of the proposed stress alarmone P1P4-bis(5'-adenosyl) tetraphosphate (Ap4A) has been examined in Vero cells. Compared with exposure to 0.8 mM-Cd2+, which causes a 30-fold increase in Ap4A, infection with simian virus 40 and poliovirus causes only a 2-fold increase, whereas herpes simplex virus type 1 results in a decrease in Ap4A during the course of the infection.

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Selected References

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  1. Baker J. C., Jacobson M. K. Alteration of adenyl dinucleotide metabolism by environmental stress. Proc Natl Acad Sci U S A. 1986 Apr;83(8):2350–2352. doi: 10.1073/pnas.83.8.2350. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Baker J. C., Jacobson M. K. Determination of diadenosine 5',5''',-P1,P4-tetraphosphate levels in cultured mammalian cells. Anal Biochem. 1984 Sep;141(2):451–460. doi: 10.1016/0003-2697(84)90070-8. [DOI] [PubMed] [Google Scholar]
  3. Baltzinger M., Ebel J. P., Remy P. Accumulation of dinucleoside polyphosphates in Saccharomyces cerevisiae under stress conditions. High levels are associated with cell death. Biochimie. 1986 Oct-Nov;68(10-11):1231–1236. doi: 10.1016/s0300-9084(86)80069-4. [DOI] [PubMed] [Google Scholar]
  4. Baril E. F., Coughlin S. A., Zamecnik P. C. 5',5'''-P1, P4 diadenosine tetraphosphate (Ap4A): a putative initiator of DNA replication. Cancer Invest. 1985;3(5):465–471. doi: 10.3109/07357908509039808. [DOI] [PubMed] [Google Scholar]
  5. Bochner B. R., Lee P. C., Wilson S. W., Cutler C. W., Ames B. N. AppppA and related adenylylated nucleotides are synthesized as a consequence of oxidation stress. Cell. 1984 May;37(1):225–232. doi: 10.1016/0092-8674(84)90318-0. [DOI] [PubMed] [Google Scholar]
  6. Coste H., Brevet A., Plateau P., Blanquet S. Non-adenylylated bis(5'-nucleosidyl) tetraphosphates occur in Saccharomyces cerevisiae and in Escherichia coli and accumulate upon temperature shift or exposure to cadmium. J Biol Chem. 1987 Sep 5;262(25):12096–12103. [PubMed] [Google Scholar]
  7. Garrison P. N., Mathis S. A., Barnes L. D. In vivo levels of diadenosine tetraphosphate and adenosine tetraphospho-guanosine in Physarum polycephalum during the cell cycle and oxidative stress. Mol Cell Biol. 1986 Apr;6(4):1179–1186. doi: 10.1128/mcb.6.4.1179. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Guédon G. F., Gilson G. J., Ebel J. P., Befort N. M., Remy P. M. Lack of correlation between extensive accumulation of bisnucleoside polyphosphates and the heat-shock response in eukaryotic cells. J Biol Chem. 1986 Dec 15;261(35):16459–16465. [PubMed] [Google Scholar]
  9. Lee P. C., Bochner B. R., Ames B. N. AppppA, heat-shock stress, and cell oxidation. Proc Natl Acad Sci U S A. 1983 Dec;80(24):7496–7500. doi: 10.1073/pnas.80.24.7496. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Lee P. C., Bochner B. R., Ames B. N. Diadenosine 5',5"'-P1,P4-tetraphosphate and related adenylylated nucleotides in Salmonella typhimurium. J Biol Chem. 1983 Jun 10;258(11):6827–6834. [PubMed] [Google Scholar]
  11. McLennan A. G., Prescott M. Diadenosine 5',5'"-P1,P4-tetraphosphate in developing embryos of Artemia. Nucleic Acids Res. 1984 Feb 10;12(3):1609–1619. doi: 10.1093/nar/12.3.1609. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Miller D., McLennan A. G. Changes in intracellular levels of Ap3A and Ap4A in cysts and larvae of Artemia do not correlate with changes in protein synthesis after heat-shock. Nucleic Acids Res. 1986 Aug 11;14(15):6031–6040. doi: 10.1093/nar/14.15.6031. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Moris G., Meyer D., Orfanoudakis G., Befort N., Ebel J. P., Remy P. Dinucleoside tetraphosphate variations in cultured tumor cells during their cell cycle and growth. Biochimie. 1987 Nov-Dec;69(11-12):1217–1225. doi: 10.1016/0300-9084(87)90149-0. [DOI] [PubMed] [Google Scholar]
  14. Ritzi E., Levine A. J. Deoxyribonucleic acid replication in simian virus 40-infected cells. 3. Comparison of simian virus 40 lytic infection in three different monkey kidney cell lines. J Virol. 1970 Jun;5(6):686–692. doi: 10.1128/jvi.5.6.686-692.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Roizman B., Spear P. G. Macromolecular biosynthesis in animal cells infected with cytolytic viruses. Curr Top Dev Biol. 1969;4:79–108. doi: 10.1016/s0070-2153(08)60481-0. [DOI] [PubMed] [Google Scholar]
  16. Weinmann-Dorsch C., Hedl A., Grummt I., Albert W., Ferdinand F. J., Friis R. R., Pierron G., Moll W., Grummt F. Drastic rise of intracellular adenosine(5')tetraphospho(5')adenosine correlates with onset of DNA synthesis in eukaryotic cells. Eur J Biochem. 1984 Jan 2;138(1):179–185. doi: 10.1111/j.1432-1033.1984.tb07897.x. [DOI] [PubMed] [Google Scholar]
  17. ZIMMERMAN E. F., HEETER M., DARNELL J. E. RNA synthesis in poliovirus-infected cells. Virology. 1963 Mar;19:400–408. doi: 10.1016/0042-6822(63)90080-1. [DOI] [PubMed] [Google Scholar]
  18. Zamecnik P. Diadenosine 5',5"'-P1,P4-tetraphosphate (Ap4A): its role in cellular metabolism. Anal Biochem. 1983 Oct 1;134(1):1–10. doi: 10.1016/0003-2697(83)90255-5. [DOI] [PubMed] [Google Scholar]

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