Table 2.
Reagents details.
| Antibodies used for Immunocytochemistry | ||||
|---|---|---|---|---|
| Antibody | Dilution | Company Cat # | RRID | |
|
| ||||
| Pluripotency Markers | Rabbit Anti-NANOG | 1:200 | Proteintech Cat# 142951-1-AP, | AB_1607719 |
| Mouse IgG2bκ Anti-OCT-3/4 | 1:200 | Santa Cruz Biotechnology Cat# sc-5279 | AB_628051 | |
| Mouse IgG1κ Anti-SOX2 | 1:200 | Santa Cruz Biotechnology Cat# sc-365823 | AB_10842165 | |
| Ectoderm Markers | Goat Anti-OTX2 | 1:200 | R&D Systems Cat# 963273 | AB_2157172 |
| Rabbit Anti-Pax6 | 1:100 | Thermo Fisher Scientific Cat# 42-6600 | AB_2533534 | |
| Endoderm Markers | Goat Anti-SOX17 | 1:200 | R&D Systems Cat# 963121 | AB_355060 |
| Rabbit Anti-Foxa2 | 1:250 | Thermo Fischer Scientific Cat# 701698 | AB_2576439 | |
| Mesoderm Markers | Goat Anti-Brachyury | 1:200 | R&D Systems Cat# 963427 | AB_2200235 |
| Rabbit Anti-Tbx6 | 1:200 | Thermo Fischer Scientific cat # PA5-35102 | AB_2552412 | |
| Secondary Antibodies | Alexa Fluor 488 Goat Anti-Mouse IgG1 | 1:1000 | Thermo Fisher Scientific #A-21121 | AB_2535764 |
| Alexa Fluor 488 Donkey Anti-Goat IgG (H + L) | 1:1000 | Thermo Fisher Scientific #A-11055 | AB_2534102 | |
| Alexa Fluor 555 Goat Anti-Rabbit IgG (H + L) | 1:500 | Thermo Fisher Scientific #A-21428 | AB_141784 | |
| Alexa Fluor 647 Goat Anti-Mouse IgG2b | 1:250 | Thermo Fisher Scientific #A-21242 | AB_2535811 | |
| Primers | ||||
| Target | Forward/Reverse primer (5′-3′) | |||
|
| ||||
| Sendai Virus | Sendai Virus genome | Mr04269880_mr | ||
| Genotyping | COL3A1 gene (c.226A > G, p.Asn76Asp) | Fwd: TTTCAAACCTTTTCAACTTTGGC Rev: CTTACTGGATCTCCCTTGGGG |
||
| House-Keeping Gene | GAPDH | HS02758991_g1 | ||
| SOX2 | HS01053049_s1 | |||
| NANOG | HS02387400_g1 | |||