Table 1. List of FLC-evolved strains with different ERG251 point mutations.

ERG251 mutations were identified in three independent FLC evolution experiments within two distinct genetic backgrounds (SC5314-derived Sn152 and BWP17, and P75063). Multiple single-colony strains from the same evolved population are organized into the same row. ERG251 point mutations are annotated on the mutated allele, and represented as allele A/allele B, except for AMS5615 where the chromosome region containing ERG251 became homozygous prior to acquisition of the point mutation. ERG251 in the P75063 background is homozygous.

Strains	Genetic background	ERG251 genotypes	Mutation types	Copy number changes	FLC MIC (24hr)	FLC SMG (48hr)
Strains from evolution experiment 1 [Zhou et al., 2024, accepted]
SN152	N.A	ERG251/ERG251	N.A	None	0.5 μg/ml	0.07
Evolved 1.1/1.2	Sn152	ERG251/ERG251 L113*	Stop gained	Chr3x3, Chr6x3, Chr5 LOH	>256 μg/ml	N. A
Evolved 2.1/2.2	Sn152	ERG251 E273* /ERG251	Stop gained	Chr3x3, Chr6x3	>256 μg/ml	N. A
Evolved 3.1/3.3	Sn152	ERG251 A60D /ERG251	Missense	Chr3x3, Chr6x3	>256 μg/ml	N. A
Evolved 3.2	Sn152	ERG251/ERG251 *322Y	Stop lost	Chr6x3	1 μg/ml	0.58
Strains from evolution experiment 2
BWP17	N.A	ERG251/ERG251	N.A	None	0.5 μg/ml	0.08
AMS5615	BWP17	ERG251/ERG251 A268D	Missense	Chr4 (partial)x4, Chr7x3, Chr7 LOH	1 μg/ml	0.79
AMS5617/5618	BWP17	ERG251 S27fs /ERG251	Frame shift	Chr4x3,Chr5 LOH	1 μg/ml	0.73
AMS5622/5623/5624	BWP17	ERG251 G62W /ERG251	Missense	Chr7x3	1 μg/ml	0.54
AMS5625/5626	BWP17	ERG251 W265G /ERG251	Missense	None	1 μg/ml	0.50
Strains from evolution experiment 3 [10]
P75063	N.A	ERG251/ERG251	N.A	None	0.5 μg/ml	0.11
AMS4130	P75063	ERG251/ERG251 H274Q	Missense	None	1 μg/ml	0.26