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. 2024 Jul 3;43(32):2475–2489. doi: 10.1038/s41388-024-03094-1

Fig. 4. BRCA2 deficiency enhances the recruitment of SLFN11 on chromatin in PARPi-treated cells.

Fig. 4

A Representative immunoblots of chromatin-bound fractions prepared from TOV-112D cells treated with 10 μM olaparib for 0, 4, 8, or 12 h. Blots were probed with the indicated antibodies. B Quantification of data from A. Data were normalized to untreated cells (0 h). Data are means ± standard deviations (n = 5–7, biological replicates). C Immunoblots of whole cell lysates prepared from the indicated TOV-112D cells. Blots were probed with the indicated antibodies. D Representative confocal microscopy images; Hoechst (blue), chromatin-bound RPA2 (red), and SLFN11 (green) in TOV-112D cells. Cells were treated with or without 10 μM olaparib for 12 h. Representative tracings of the distribution of signals along the white dashed arrow (a and b) are shown in the merged panel. E Quantification of data from D. Scatter plots show mean signal intensities of RPA2 and SLFN11. Data are means ± standard deviations (n = 104–195, one-time experiment). NS not significant, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001 (one-way analysis of variance with Tukey’s post-hoc multiple comparisons test).