Fig. 6. Intestinal Nogo-B knockout alleviates STZ-induced T2DM.
a Experimental design in vivo. Nogof/fVillinCre (STZ-NVK group) and Nogof/f (STZ group) mice received STZ and fed on HFD to induce T2DM, using the Nogof/f mice as healthy control (NC group). b Body weight of mice in NC, STZ, and STZ-NVK groups (n = 6 mice per group). c Fasted glucose levels of mice in NC, STZ, and STZ-NVK groups (n = 6 mice per group). d–f Levels of insulin, glucagon and GLP1 of mice in NC, STZ, and STZ-NVK groups (n = 6 mice per group). g OGTT and AUC analysis of mice in NC, STZ, and STZ-NVK groups (n = 6 mice per group). h ITT and AUC analysis of mice in NC, STZ, and STZ-NVK groups (n = 6 mice per group). i GSIS and AUC analysis of mice in NC, STZ, and STZ-NVK groups (n = 6 mice per group). j Representative images of immunofluorescence staining (GLP1, green; DAPI, blue) of small intestine from mice in NC, STZ, and STZ-NVK groups. White arrowheads indicate GLP1-positive signals. Quantification of results were expressed as the mean numbers of GLP1-positive cells in 5 HPFs (n = 5 mice per group, right panel). k Representative images of immunofluorescence staining (PCSK1, green; proGCG, red; DAPI, blue) of small intestine from mice in NC, STZ, and STZ-NVK groups. a–c The intensity distribution of the marker scans in the boxed area shows the spatial distribution and fluorescence intensity of the different markers. Enhanced co-localization is indicated by the resemblance in shape of the intensity distribution curves and the increased number of overlaps. The experiment was conducted independently on three occasions. l Islet images of mice in NC, STZ, and STZ-NVK groups (glucagon, green; insulin, red; DAPI, blue). Analysis of β-cell or α-cell as the percentage of pancreatic cells (n = 5 mice per group, bottom panel). Data are expressed as the mean ± SEM. The p values were calculated by one-way ANOVAs. The p values of (b, c, g, h) were indicated STZ-KD group vs. STZ group. Source data are provided as a Source Data file.