Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2024 Jul 1;25(8):3348–3372. doi: 10.1038/s44319-024-00183-w

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. Creative Commons Public Domain Dedication waiver http://creativecommons.org/publicdomain/zero/1.0/ applies to the data associated with this article, unless otherwise stated in a credit line to the data, but does not extend to the graphical or creative elements of illustrations, charts, or figures. This waiver removes legal barriers to the re-use and mining of research data. According to standard scholarly practice, it is recommended to provide appropriate citation and attribution whenever technically possible.

PMC Copyright notice

(A) Linkage map showing the sequence position and cross-linked residue pairs between the different Mis18_core complex subunits, Mis18α, Mis18β and Mis18BP1_20-130. Left panel highlights cross-linked residues between Mis18α and Mis18β. Black lines highlight cross-links between N-terminal α-helix of Mis18α and C-terminal helical regions of proteins. Right panel highlights cross-links observed between (i) Mis18BP1_20-130 and Mis18α (purple), (ii) Mis18BP1_20-130 and Mis18β (light pink), (iii) Mis18BP1_20-130 self cross-links (light grey). White boxes represent residual residues left over from tag cleavage. Dark boxes show Yippee domains and regions of α-helices. (B) Model of the Mis18_core complex generated using partial structures determined using X-ray crystallography and AlphaFold2 (Jumper et al, 2021) and cross-linking restrained molecular docking in EM maps. Mis18BP1 shown in salmon, Mis18α in purple and Mis18β in light pink. (C) Histograms show the percentage of satisfied or violated cross-links for structures modelled using MODELLER (Sali and Blundell, 1993). (D) Representative images of 2D classes from Mis18_core particles picked using CryoSPARC (Punjani et al, 2017). Scale bar shows 100 Å. (E) Model (Class I) generated for Mis18_core from negative staining EM analysis. This shows that the overall shapes of the Mis18_core resemble a telephone handset with ‘ear’ and ‘mouth’ pieces. Arrows denote the different orientations shown. (F) Theoretical SAXS scattering curves of Mis18α/β ΔN model compared to experimental data.