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. 2024 Aug 10;7:972. doi: 10.1038/s42003-024-06655-y

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License, which permits any non-commercial use, sharing, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if you modified the licensed material. You do not have permission under this licence to share adapted material derived from this article or parts of it. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by-nc-nd/4.0/.

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Fig. 3 — A PDMS mold with uniform microwells. B The schematic presentation of agarose microwells on PDMS and the microspheroids formation in microwells. C Representative bright-field images of DPSC to microspheroids in a time-dependent manner. Representative confocal z-projection images of cell cytoskeleton in microspheroids stained by Phalloidin actin (D), and Live/Dead cells in microspheroids stained by Calcein AM/PI. Red arrows were pointed to the dead cells (E). F The diameter of microspheroids over time. Scale bar 50 µm. The data are presented as mean ± SD (n = 3). OM osteogenic medium.