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. 2024 Aug 10;7:972. doi: 10.1038/s42003-024-06655-y

Table 1.

Primers used in RT-qPCR to examine gene expression

Gene Acc. No Primer sequence (5′-3′) Product length (bp)
ALP NM_001127501.4

F: GGACCATTCCCACGTCTTCAC

R: CCTTGTAGCCAGGCCCATTG

137
BMP2 NM_001200.4

F: GGGAGAAGGAGGAGGCAAAGA

R: CTGGGGAAGCAGCAACGCTA

190
RUNX2 NM_001015051.4

F: ACCCAGAAGGCACAGACAGAAG

R: AGGAATGCGCCCTAAATCACT

82
OCN NM_199173.5

F: CTCACACTCCTCGCCCTATTGG

R: GTAGCGCCTGGGTCTCTTCACT

147
OPN NM_000582.2

F: GCTAAACCCTGACCCATC

R: CTTTCGTTGGACTTACTTGG

72
COL-I NM_000088.4

F: CAGTGGTAGGTGATGTTCTGGGAG

R: CAAGAGGCATGTCTGGTTCGG

150
WNT6 NM_006522.4

F: GTGCAACTGCACAACAACGAGG

R: GAAATGGAGGCAGCTTCTGCCA

127
GAPDH NM_001357943.2

F: GGACCTGACCTGCCGTCTAG

R: GTAGCCCAGGATGCCCTTGA

100