Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2024 Jun 27;25(8):3406–3431. doi: 10.1038/s44319-024-00186-7

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. Creative Commons Public Domain Dedication waiver http://creativecommons.org/publicdomain/zero/1.0/ applies to the data associated with this article, unless otherwise stated in a credit line to the data, but does not extend to the graphical or creative elements of illustrations, charts, or figures. This waiver removes legal barriers to the re-use and mining of research data. According to standard scholarly practice, it is recommended to provide appropriate citation and attribution whenever technically possible.

PMC Copyright notice

(A, B) IHC-based quantification of immune cell infiltration and PD-L1 expression in tumors from Fib^Ctrl and Fib^ΔZeb1 mice in the AOM/DSS model (A) and after orthotopic transplantation of AKP tumor organoids (B). Number of experimental mice per genotype are indicated (CD4, CD8, FOXP3, F4/80, B220, PD-L1: P = 0.0414, 0.2745, 0.0005, 0.3751, 0.0134, 0.0082 (AOM/DSS), P = 0.3383, 0.0068, 0.5852, 0.0401, 0.0122, 0.0545 (orthotopic), Student’s t test). (C) qRT-PCR analysis of Cxcl1 and Ccl2 mRNA expression in fibroblasts after in vitro recombination of Zeb1 and stimulation with IL1α (n = 4, two independent fibroblast lines per genotype in two biological replicates, 0, 5, 24 h: P = 0.9248, 0.0070, 0.1816 (Ccl2), P = 0.9998, 0.0337, 0.0890 (Cxcl1), Šídák’s multiple comparisons test). (D) Western blot of NFκB pathway activity in fibroblasts after 15 min of IL1α stimulation. β-ACTIN detection was used as a loading control. (E) IHC-based quantification of phospho-NFκB p65 (Ser536) in mice after AOM/DSS tumorigenesis or transplantation of AKP^re organoids. Numbers of experimental mice per genotype are indicated (AOM/DSS: P = 0.0435, orthotopic: P = 0.0238, Student’s t test). (F) Quantification of T-cell attraction to fibroblasts after in vitro recombination of Zeb1 (n = 7 independent lines, no Fib vs Fib^Ctrl: P = 0.0014, no Fib vs Fib^ΔZeb1: P < 0.0001, Fib^Ctrl vs Fib^ΔZeb1: P = 0.0043, Tukey’s multiple comparisons test). (G–I) Fib^Ctrl and Fib^ΔZeb1 fibroblasts were seeded in 3D ECM and expression of fibroblast subtype markers was quantified by qRT-PCR. Baseline marker expression of Fib^Ctrl and Fib^ΔZeb1 fibroblasts without additional treatment (G) and after treatment with IL1α (H) or TGFβ (I) relative to the respective untreated condition (n = 9/6 for Fib^Ctrl/Fib^ΔZeb1 fibroblast lines, Ccl2, Cxcl1, Acta2, Tagln: P = 0.6830, 0.8861, 0.0184, 0.0084 (baseline), P = 0.9318, 0.5463, 0.3182, 0.7161 ( + IL1α), P = 0.0670, 0.0077, 0.1469, 0.0346 ( + TGFβ), Student’s t test). Data information: Data are presented as mean ± SD (A–C, E–I). Source data are available online for this figure.