Fig. 2.
Position of target sites of the sgRNAs in SlDND1 (Solyc02g088560.4.1) and editing details of CRISPR events. (a) Graphical representation showing the locations of the sgRNAs’ target sites in pink, primers flanking all target sites in blue, and primers for amplification of smaller overlapping regions in green. The 3071-bp region of SlDND1 (fragment A) containing 4 sgRNAs was divided into fragments named B, C, D, E, and F for Sanger sequencing and identification of the mutations. (b) Mutations in TF2 families from 3 different editing events. The results were obtained from Sanger sequencing