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. 2024 Aug 9;25:777. doi: 10.1186/s12864-024-10684-6

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License, which permits any non-commercial use, sharing, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if you modified the licensed material. You do not have permission under this licence to share adapted material derived from this article or parts of it. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by-nc-nd/4.0/.

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Fig. 5 — Regulation of chemotaxis and motility in Xanthomonas strains expressing hrpG*. A: Regulation of orthogroups (OG) involved in chemotaxis and motility. Only significantly DEGs (AdjPval < 0.05) are colored. Missing orthologs are marked in gray. Orthogroup annotation is given on top of the figure, orthogroup number at bottom of the figure. Due to the extensive number of orthogroups potentially involved in chemotaxis, only those orthogroups that are regulated by HrpG* in at least two strains are shown, except for the secondary flagellar cluster specific to Xcm strains. Additionally, for both the flagellar and pilus gene clusters, only the regulation of key representative genes that encode various structural components of the flagella and pili are shown. MCPs: methyl-accepting chemotaxis proteins. B: Motility assay showing swimming abilities after 48 h on 0.3% agar plates for four Xanthomonas strains. The plot represents the ratio of swimming halo of HrpG* mutant against wild-type (WT) strain for three biological replicates. Representative pictures are shown