FIG. 4.

Accumulation of viral RNAs and proteins in N. benthamiana plants and protoplasts inoculated with CymRSV/CIRV chimeras. (A) Symptoms of N. benthamiana inoculated with the viruses indicated. The photos were taken 4 wpi. (B) Western blot analysis of the accumulation of p19 (upper panel) and p33 (lower panel) in virus-infected plants. (C) Northern analysis of viral RNAs extracted from infected N. benthamiana plants (lower panel) and protoplasts (upper panel). Samples for protein and RNA extractions were taken from upper leaves showing the first appearing systemic symptoms (typically 7 to 10 dpi) and from protoplasts harvested 24 hpi.