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. 2024 Aug 10;15:6860. doi: 10.1038/s41467-024-50846-4

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — Alignment and dense segmentations of two cutout volumes of the same area in hippocampus CA3 region imaged with bdTEM. Results of 1 independent experiment. a–d EM overview and segmentation of a cutout volume imaged with 120 ms exposure time (191 × 151 × 2.48 µm^3,, 55 sections); e Segmentation of a cutout volume imaged with 40 ms exposure time (191 × 151 × 0.9 µm³, 20 sections).