Table 1.

Performance metric for TEM with Cricket

imaging parameters	avg. 3 nm/pix (120 ms exp.)	avg. 3 nm/pix (40 ms exp.)	peak 3 nm/pix (40 ms exp.)	avg. 3.6 nm/pix (40 ms exp.)	unit
x,y pixel size*	3	3	3	3.6	nm/pixel
tile size (per size)	6000	6000	6000	6000	pix
FOV size	18	18	18	21.6	μm
pixels per tile	36	36	36	36	Mpixel
supertile size (per side, 15% overlap b/w tiles)	16200	16200	16200	16200	pix
overlap between supertiles	600	600	600	600	pix
exposure time (exp.)	120	40	40	40	ms
tiles per section (w/overlap)	4356	4356	4140	2916	tile
supertiles per section (w/overlap)	484	484	460	324	supertile
imaging time per section	11.7	6.6	4.7	4	minute
transition overhead (tape translation, ROI definition, autofocus, etc.)	2.2	2.2	1.8	2.2	minute
total time per section	13.9	8.8	6.5	6.2	minute
burst imaging rate (imaging only)	300	900	900	900	Mpix/s
montage imaging rate (w/ stage, Cricket)	223	397	529	437	Mpix/s
net imaging rate (w/ stage, Cricket, transition)	188	297	382	282	Mpix/s
effective imaging rate (w/ stage, Cricket, transition, overlap)	142	224	288	213	Mpix/s
sections per day (1 scope at 24 h)	103	163	221	232	section
time to image 1 mm³ (4 scopes at 24/7)	54	35	26	24	days
time to image 1 mm³ (4 scopes at 65% uptime #)	83	53	39	37	days

Based on 1 mm² section of 45 nm thick.

# 65% uptime accounts for microscope maintenance, imaging pause (Yin et al. 2020).

*Pixel size is different from resolution, which refers to the capability of an imaging system to perceive two point sources separately.

Typical TEM section thickness is 45 nm in z, while FIB-SEM offers a z resolution of 4 nm (Xu et al. 2017).