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. 2024 Aug 10;11:868. doi: 10.1038/s41597-024-03697-z

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — Experimental workflow. (a) The screen methodology is depicted starting with the primary screen, including staining, imaging, analysis and hit ranking, followed by a secondary screen of hits and bioinformatic shortlisting. Representative images of each stain are depicted, with CKI (CDK inhibitors) representing p21 or p27. The image analysis pipeline is represented by screenshots from Harmony (PerkinElmer) software. (b) Flow chart depicting how the secretome library was refined at each stage to a final list of proteins which induce G0 arrest.