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2D vascularized liver organoids. (a) Workflow illustration. (b) The phase contrast images of the primary liver cells right after digestion and those cultured in vitro in regular dishes for 4 days. The white arrow pointed to a microvessel. (c–e) Immunostaining of the 2D vascularized liver organoids cultured for 4 days in vitro. The antibodies were against CD31, E-cadherin, and Desmin. DAPI stained nuclei. Scale bars, 100 µm.
