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. 2024 Aug 10;15:20417314241268344. doi: 10.1177/20417314241268344

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© The Author(s) 2024

This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).

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Figure 6. — Integrins in vascularized liver organoids. (a) The qPCR analysis of vascularized liver organoids on the ECM of different stiffness. N = 3. One-way or two-way ANOVA was performed on the data, followed by Bonferroni post hoc tests. p < 0.05 was considered significant. (b) Immunostaining of vascularized liver organoids cultured on 3 GPa ECM in the control medium by the antibodies against CD31, E-cadherin, and p-ITGB1 (Y783). DAPI stained nuclei. Arrows pointed to CD31⁺ p-ITGB1⁺ microvascular endothelial cells. Arrowheads pointed to E-cadherin⁺ p-ITGB1⁺ epithelial cells. Scale bars, 100 µm. The cell culture time was 1 week.