Figure 2.

NER-deficient animals show somatic formaldehyde sensitivity, which is further enhanced by loss of ADH-5. (A) Experimental scheme (see Materials and methods for details). (B) Survival of NER mutants upon formaldehyde (FA) after 48 h and (C) after 72 h on plate. (D) Developmental timing of NER mutants upon FA after 48h on plate and (E) after 72 h on plate. (F) Survival of NER mutants in an adh-5 mutant background upon FA after 72h on plate (for N2 control the survival at 5 mM is 91.5 ± 1.9% as shown in Figure 2C). (G) Developmental timing of NER mutants in an adh-5 mutant background upon FA after 72 h on plate. Each condition was performed in three technical replicates, and the significance was determined via two-way ANOVA for survival, followed by Tukey's multiple comparison test, for which is defined *P < 0.05, **P< 0.01, ***P < 0.001, ****P < 0.0001. (H) Top, scheme depicting the experimental set up; bottom viability determined 72 h after the 4 h-FA pulse (n = 6, ****P< 0.0001 for a two-tailed t-test between the groups indicated in the figure). (I) DNA–protein crosslink (DPC) accumulation after a 4 h-FA pulse determined 24 h upon recovery for GM00637 (healthy fibroblasts, left); and for GM16095 (CSBmut fibroblasts, right) (n = 6, mean ± SEM). One-way ANOVA corrected with Dunnett test for multiple comparison (*P= 0.0109, ****P< 0.0001; *P= 0.0242, ***P= 0.0003). ANOVA tables for panels B, C, D, E and G can be found as supplementary tables. Cartoons were created with BioRender.com.