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. 2024 Jun 19;52(14):8271–8285. doi: 10.1093/nar/gkae519

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© The Author(s) 2024. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 3. — Fecundity is reduced in NER mutants upon formaldehyde (FA) toxicity, which is further enhanced upon loss of ADH-5. (A) Experimental scheme (see Materials and Methods for details). Panels (B–E) show the number of eggs produced by three animals within 3 h for strains carrying mutations for (B) xpc-1, (C) csb-1, (D) xpc-1;csb-1 and (E) xpa-1. Panels (F–I) display the hatching rate as counted 24 h post-egg-laying for (F) xpc-1, (G) csb-1, (H) xpc-1;csb-1 and (I) xpa-1. The graphs summarize the data of four technical replicates, and the significance was determined via the two-way ANOVA, followed by the Tukey's multiple comparison test, for which counts *P< 0.05, **P< 0.01, ***P< 0.001, ****P< 0.0001. ANOVA tables for panels B–I can be found as supplementary tables. Cartoons were created with BioRender.com