Figure 3.

Measurement of transcriptional potential of a specific dimer pair. C3H10T1/2 cells were transfected with the indicated constructs in addition to the promoter-less renilla luciferase construct in triplicate per condition. The cells were harvested and subjected to luciferase reporter gene assay. A constant volume of cell lysate was used to determine reporter gene activity (firefly luciferase) and transfection control (renilla luciferase), which was used for standardizing the transfection efficiency. An average of the standardized firefly luciferase values of each condition in triplicate was calculated and fold activation (FA) cpmpared to control condition was graphed. Error bar = standard deviation, n = 3.