Figure 2.

Characterization of HGTs within the human gut microbiome. (A) Distribution of the number of HGT breakpoint pairs in different samples. The black dashed line indicates the median value. (B) Phylogenetic tree of the 842 genomes involved in HGT in no less than 10% of the samples. Colors indicate the corresponding phylum of each genome. The height of the inside bar represents the average HGT frequency among samples. (C) HGT frequency of different taxa at the phylum, class, order, family, genus, and species levels. Only the top five taxonomic units are shown for each taxonomic level, while the remaining taxa are labeled as ‘other’. (D) Schematic representation of the reconstructed local genome using the inferred HGT event, reads alignment visualized with UGENE (89). (E) Distribution of the length of transferred sequences in HGT events. The black dashed line indicates the median length. (F) Distribution of transferred sequences with various numbers of target insertion sites. (G) Illustration of an example showing a transferred sequence has three target insertion sites within the recipient genome. The blue arrow represents the transferred sequence. The transferred sequence is inserted into the recipient genome twice in a reverse complement manner. (H) Intra-taxa HGT proportion at different taxomomic levels. The red line indicates a proportion of 50%. (I) HGT frequency of the genome pairs at different phylogenetic distance levels. The x-axis indicates the phylogenetic distance bins and the y-axis shows the HGT frequency.