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. 2024 Jun 25;52(14):8286–8302. doi: 10.1093/nar/gkae526

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© The Author(s) 2024. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 3. — The nuclear basket regulates the expression of the SUMO SENP protease Ulp1. (A) Left panel: expression of Ulp1-GFP in indicated strains. An untagged WT strain was included as control for antibody specificity. Tubulin was used as a loading control. Right panel: quantification. The normalized amount of Ulp1 was calculated by dividing the GFP signal by tubulin signal. The normalized amount of Ulp1-GFP in the mutants is indicated as a percentage of the WT. Dots represent values obtained from independent biological experiments. P value was calculated by two-sided t-test (**** P ≤ 0.0001). (B) Example of fluorescence (bottom panel) and bright-field images (top panel, DIC) of cells expressing the endogenous Ulp1-GFP fusion protein in indicated strains. Scale bar 5 μm. (C) Expression of SUMO conjugates in indicated strains. A strain deleted for pmt3 gene that encodes the SUMO particle (sumoΔ) was added as control for antibody specificity. * indicates unspecific signal.