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. 2024 Jun 25;52(14):8286–8302. doi: 10.1093/nar/gkae526

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© The Author(s) 2024. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 5. — Tethering of Ulp1 to the RFB rescues RDR defect in nup60Δ but not in alm1Δ cells. (A) Frequency of RFB-induced RS in indicated strains and conditions. Dots represent values obtained from independent biological experiments. Red bars indicate mean values ± SD. P value was calculated by two-sided t-test (**** P ≤ 0.0001; ns: non-significant). (B) Quantification of co-localization events in S-phase cells in indicated conditions and strains. Dots represent values obtained from independent biological experiments. At least 100 nuclei were analyzed for each strain and condition. P value was calculated by two-sided Fisher's exact test (**** P ≤ 0.0001; *** P ≤ 0.001; ns: non-significant). (C–E) Frequency of RFB-induced RS in indicated strains and conditions. Dots represent values obtained from independent biological experiments. Red bars indicate mean values ± SD. P value was calculated by two-sided t-test (**** P ≤ 0.0001; ** P ≤ 0.01,* P ≤ 0.05 ns: non-significant).