Table 1.
Kinetics constants (kon and koff), calculated dissociation equilibrium constants (KD) and apparent selectivity for two variants of iMab antibody interacting with hTeloC-27 sequence at pH 6.0 and 7.5a
| iMab variant | pH 6.0 | pH 7.5 | Apparent selectivityb | ||||
|---|---|---|---|---|---|---|---|
|
k
on
(103 M−1 s−1) |
k
off
(10−4 s−1) |
K
D
(nM) |
k
on
(103 M−1 s−1) |
k
off
(10−4 s−1) |
K
D
(nM) |
||
| scFv-His6 | 3.7 (2.4; 5.0) | 1.2 (1.1; 1.2) | 34 (24; 44) | 2.3 (1.7; 3.0) | 2.4 (2.0; 2.8) | 108 (63; 153) | 3.2 |
| scFv-His6-FLAG | 11.8 (11.4; 12.4) | 3.3 (2.6; 3.7) | 28 (23; 31) | 13.0 (8.0; 18) | 8.2 (3.3; 13) | 91 (18; 164) | 3.3 |
aData are arithmetic means from several independent measurements; the individual values are given in parentheses.
bApparent selectivity = KD (at pH 7.5)/KD (at pH 6.0).