Table 2.
Kinetic constants (kon and koff) and calculated dissociation equilibrium constants (KD) for interaction of iMab (scFv-His6-FLAG) with the native and the constrained hTeloC substrates at pH 6.5 and 20 °Ca
| Substrate | k on (103 M−1 s−1) | k off (10−4 s−1) | K D (nM) |
|---|---|---|---|
| hTeloC-27 | 11.2 (11.0; 11.4) | 6.1 (5.4; 6.7) | 54 (49; 58) |
| Constrained hTeloC (I) | 4.6 (4.4; 4.9) | 7.2 (6.8; 7.6) | 155 (140; 171) |
aData are arithmetic means from two independent measurements; the individual values are given in parentheses.