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. 2024 Jul 29;15:1440662. doi: 10.3389/fimmu.2024.1440662

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Copyright © 2024 Barreto, Neves, Cardoso, Meira, Daltro, Figueira, Santos, Silva, Távora, Neto, Macambira, Lampe, Coutinho, Kasai Brunswick, Ribeiro dos Santos, Campos de Carvalho and Soares

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Distribution of total Cx43, Cx43^S368, and Cx43^S325/328/330 in human cardiac muscle tissue. (A, C, E) Sections of explanted hearts from non-Chagas patients and (B, D, F) sections of explanted hearts from Chagas patients who underwent heart transplantation were analyzed by immunofluorescence. Samples were labeled with WGA (red), cell nuclei with DAPI (blue), and total Cx43 total, Cx43^S368 or Cx43^S325/328/330 (green). (G, H) Co-labeling of Cx43 (green) and N-cadherin (red), and co-localization between Cx43 and N-cadherin (yellow). White arrows indicate the presence of Cx43 outside the intercalated disc area. Scale bars = 50 µm. WGA, Wheat Germ Agglutinin; N-cad, N-cadherin; Cx43, Connexin 43.