Figure 2.

Ribosome hibernation factors can occupy ribosomal drug-binding sites. (A) Schematic illustration compares the ribosomal binding sites for the hibernation factors RMF and HPF, and for tRNAs (labeled as E, P, and A), mRNA, and ribosome-targeting antibiotics that bind to the decoding center (DC-drugs) and the ribosomal mRNA channel. (B) Close-up view illustrating the overlap between the ribosomal binding sites for hibernation factor HPF and several families of ribosome-targeting antibiotics. (C) Schematic illustration of the location of the binding site of Balon relative to the binding sites of ribosome-targeting antibiotics (labeled as PTC-drugs and DC-drugs to indicate their binding to the peptidyl-transferase center and the decoding center of the ribosome, respectively). (D,E) Close-up views illustrating the overlap between the ribosomal binding sites for hibernation factor Balon and several families of ribosome-targeting antibiotics in the small ribosomal subunit (D) and the large ribosomal subunit (E).