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. 2024 Aug 11;10:359. doi: 10.1038/s41420-024-02132-x

Fig. 3. Effect of rhMFGE8 application or MGFE8 siRNA on hippocampal neurogenesis after SAH.

Fig. 3

A Experimental design, animal groups and time frame of the analyses in experiment 2. B Double immunofluorescence staining with DCX (red) and BrdU (green) in hippocampus between sham, SAH + vehicle and SAH + rhMFGE8 group as well as SAH + Scr siRNA, SAH + MFGE8 siRNA group. Arrows indicated the DCX positive and BrdU positive cells. C Quantitative analyses of DCX positive BrdU positive cells in three groups. The DCX positive BrdU positive cells are decreased in SAH+vehicle group compared to sham, which are reversed by rhMFGE8 treatment. The cells are also decreased in SAH + MFGE8 siRNA group compared to SAH + Scr siRNA group. The Red box indicated the hippocampus in the brain slice. **P < 0.01 vs sham group, ##P < 0.01 vs SAH + vehicle group, $$P < 0.01 vs SAH + Scr siRNA group. Error bars were represented as mean ± SD. One-way ANOVA, Tukey’s test, n = 3 per group. Scale bar, 100 μm. BrdU bromodeoxyuridine.