Table 1.
Primer sequences used for qPCR in hCMEC/D3 cells
| Target genes | Forward primer sequences | Reverse primer sequences |
|---|---|---|
| GAPDH | 5'-CAG GAG GCA TTG CTG ATG AT-3' | 5'-GAA GGC TGG GGC TCA TTT-3' |
| AGRN | 5'-CCG CCA GGA GAA TGT CTT CAA-3' | 5'-TTT CGT AGG TGA CTC CGT CGT-3' |
| NAMPT | 5'-AGC AGA ACA CAG TAC CAT AAC A-3' | 5'-CCC ATA TTT TCT CAC ACG CAT T-3' |
| RPS27A | 5'-GGA CGT ACT TTG TCT GAC TAC A-3' | 5'-TAT TTC AGG ACA GCC AGC TTA A-3' |
| GGT5 | 5'-GTC AGC CTA GTC CTG CTG G-3' | 5'-GGA TGG CTC GTC CAA TAT CCG-3' |
| ARHGEF17 | 5'-TCC CTG TCA AAT CCA GAT ATC G-3' | 5'-GGA TGG GTA TGG GTT CAA GTT-3' |
| PLOD1 | 5'-AAG CCG GAG GAC AAC CTT TTA-3' | 5'-GCG AAG AGA ATG ACC AGA TCC-3' |
| MRC2 | 5'-CGT AGG GTT CTC TTA CCA CAA T-3' | 5'-GTG CTC AAA GAA CTT GTA CTC G-3' |
| TECR | 5'-AAG ACC CAT CCG CAG TGG TA-3' | 5'-CGT ACT CTG TTA GGA AGA CCG TC-3' |
| EFHD2 | 5'-GAT GTT CAA GCA GTA TGA TGC CGG-3' | 5'-AGA TCA GGA GGA ACT CCC GG-3' |
| MCAM | 5'-GTC AAT TTA ACC ACC CTC ACA C-3' | 5'-GCT TGC CCT TCT TAT AGA GGA A-3' |
| LRP1 | 5'-GTC TAC CAT CAC ACC TAC GAG-3' | 5'-CAG ACT GAG GGA GAT GTT GAT G-3' |
AGRN: Agrin; ARHGEF17: Rho guanine nucleotide exchange factor (GEF) 17; GAPDH: glyceraldehyde-3-phosphate dehydrogenase; GGT5: gamma-glutamyltransferase 5; LRP1: low density lipoprotein receptor related protein 1; MCAM: melanoma cell adhesion molecule; MRC2: mannose receptor C type 2; PLOD1: procollagen lysine-1,2-oxoglutarate-5-dioxygenase 1; qPCR: quantitative polymerase chain reaction; RPS27A: ribosomal protein S27A.